transit -2020 (Mirus Bio)

Mirus Bio is a verified supplier

Mirus Bio manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Mirus Bio transit -2020
Transit 2020, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 97/100, based on 1567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transit+2020/TransIT+-2020/custom%40mir-5400%4042663800
Average 97 stars, based on 1567 article reviews

transit -2020 - by Bioz Stars, 2026-10

97/100 stars

Images

Plasmid Preparation:

Article Title: ADH1C Down-Regulation Is a Key Hypoxia Response in Colon Epithelium
Article Snippet: .. A lentiviral vector was mixed with the three packaging plasmids in a ratio of 6:5, and 15 μg of the mix was used to transfect one 10-cm cell culture plate of approximately 25% confluent HEK293T cells using 30 μL (ratio 1:2) of TransIT-2020 (Mirus, Madison, WI; number MIR5404) following the manufacturer's protocol. .. Viral particles in the supernatant were harvested after 2 to 3 days, concentrated using Lenti-X Concentrator (Takara; number 631231) following the manufacturer's protocol, and suspended in 1/100 th of the original harvested volume in WENR medium, then stored at –80°C.

Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor
Article Snippet: For dose–response BRET 2 assays, 0.1 million HEK293T cells (American Type Culture Collection (ATCC), CRL-11268; Mycoplasma free; authenticated by the supplier) were seeded in six-well plates 24 h before transfection. .. Plasmid DNAs (500 ng of SST3–TAS2R43, 200 ng of Gα i1 –Rluc or Gα gust –Rluc, 100 ng of Gβ 3 and 100 ng of GFP2–Gγ 9 or GFP2–Gγ 1 ) were mixed with TransIT-2020 (MirusBio) in Opti-MEM (Gibco) and added to HEK293T cells cultured in DMEM containing 10% FBS, 1 IU per ml penicillin G and 100 μg ml −1 streptomycin. .. After 24 h, transfected cells were washed with PBS, trypsinized and then plated at 10,000 cells per well onto poly( L -lysine)-coated 384-well white, clear-bottom plates (Greiner) in DMEM containing 1% dialyzed FBS, 1 IU per ml penicillin G and 100 μg ml −1 streptomycin.

Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor.
Article Snippet: For dose–response BRET2 assays, 0.1 million HEK293T cells (American Type Culture Collection (ATCC), CRL-11268; Mycoplasma free; authenticated by the supplier) were seeded in six-well plates 24 h before transfection. .. Plasmid DNAs (500 ng of SST3–TAS2R43, 200 ng of Gαi1–Rluc or Gαgust–Rluc, 100 ng of Gβ3 and 100 ng of GFP2–Gγ9 or GFP2–Gγ1) were mixed with TransIT-2020 (MirusBio) in Opti-MEM (Gibco) and added to HEK293T cells cultured in DMEM containing 10% FBS, 1 IU per ml penicillin G and 100 μg ml−1 streptomycin. .. After 24 h, transfected cells were washed with PBS, trypsinized and then plated at 10,000 cells per well onto poly(L-lysine)-coated 384-well white, clear-bottom plates (Greiner) in DMEM containing 1% dialyzed FBS, 1 IU per ml penicillin G and 100 μg ml−1 streptomycin.

Cell Culture:

Transfection:

Article Title: Structure of the melatonin–related orphan receptor, GPR50
Article Snippet: .. Transfections were performed using TransIT-2020 (Mirus Bio) at a 1:3 (w/w) DNA-to-transfection reagent ratio in OptiMEM (Gibco). .. The following day, the cells were harvested and seeded into poly- L -lysine-coated 96-well plates at a density of 40,000 to 50,000 cells per well in DMEM supplemented with 1% dialyzed FBS (Gibco) and incubated for 12 hours.

Article Title: Serotonergic Polypharmacology of 2-Halogenated Tryptamines
Article Snippet: Cell lines were subcultured in high-glucose DMEM (VWR) supplemented with 10% FBS (Life Technologies). .. All transfections were prepared in Opti-MEM (Invitrogen) and used a 3:1 ratio of TransIT-2020 (Mirus) μL/μg total DNA. .. Next day, cells were plated at an approximate density of 40-50,000 cells per well into poly-L-lysine-coated 96-well white assay plates (Grenier Bio-One).

Article Title: Mechanistic insights into the therapeutic properties of delta opioid receptor
Article Snippet: GraphPad Prism 10.1.1 software was used to analyze the results. .. HEK-293 T cells (ATCC) were transfected with a mixture of HA-DOR-GFP DNA (780 ng in a T25 flask) and TransIT 2020 (1 ng:2 μL ratio, Mirus Bio) in Opti-MEM. ..

Article Title: Cryo-EM Structures of Brain-Derived G Protein-Coupled Receptors: The First Direct Visualization from Mammalian Brain Tissue
Article Snippet: .. After 24 h, cells were transfected with TransIT-2020 (Mirus Bio; 3 μl μg−1 DNA). .. For each condition, 250 ng of each plasmid—mouse Grm2 (mGluR2) or mouse Grm3 (mGluR3), human SLC1A1 (EAAT3), GαoA-RLuc8, Gβ3 and Gγ8-GFP2—was combined in Opti-MEM (Thermo Fisher Scientific) to form the transfection complex.

other:

Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: The following antibodies and reagents were used at the indicated concentrations (IHC-immunohistochemistry, ICC-immunocytochemistry, IB-immunoblot): anti-HA rabbit (Cell Signaling Technologies, Cat# 3724, 1:1,000, ICC, 1:2,000 IB), anti-Homer1 rabbit (Synaptic Systems, Cat#160003, 1:500 IHC), anti-Bassoon mouse (AbCam, Cat#ab82958, 1:500 IHC,1:1,000 ICC), anti-SHANK2 guinea pig (Synaptic Systems, Cat#162204, 1:1,000 ICC, 1:500 IHC), anti-Syn1/2 rabbit (Synaptic Systems, Cat#106002, 1:500 IHC), anti-VGAT guinea pig (Synaptic Systems, Cat#131004, 1:500 IHC), anti-Synaptoporin rabbit (Synaptic Systems, Cat#102002, 1:500 IHC), anti-Gephyrin mouse (1:2,000 ICC, Synaptic Systems, Cat#147111) anti-Parvalbumin rabbit (SWANT, Cat#PV27a, 1:2,000 IHC), anti-Synaptotagmin-2 (Synaptic Systems, Cat#105225,1:500 IHC), anti-GAD67 mouse (Millipore Sigma, Cat#AB5062P, 1:1,000 ICC), anti-βactin mouse (Sigma, Cat#A1978, 1:10,000 IB) and corresponding fluorescently-conjugated goat secondary antibodies from Life Technologies (1:1,000).

Review